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Cd distribution in the tissues of oysters Crassostrea gigas exposed chronically in situ more. View on direct. Metallothionein concentration in sponges Spongia officinalis as a biomarker of metal contamination more.

The synthesis of metallothioneins MTs is often induced when organisms are exposed to heavy metals in the field. They are among the major With a view to include MTs in biomonitoring programs, the organisms most commonly studied are bivalves.

The detection of large quantities of metals in sponges suggests the existence of detoxification systems and indeed, the presence of metallothionein-like proteins MTLPs has been reported in two different species of sponges.

In Spongia officinalis, the present study has demonstrated the presence of compounds exhibiting most of the characteristics of MTs: cytosolic, heat-stable, with apparent molecular mass of 4 to 15 kDa and binding at least Ag, Cu and Zn.

Specimens have been collected along the French Mediterranean coast from three sites differing by their degree of contamination.

Relationships between MTLP and metal concentrations have been established. For copper, mercury and zinc, the correlations were significantly positive.

Importance of metallothioneins in the cadmium detoxification process in Daphnia magna more. Good knowledge of the relationship between toxic metals and biological systems, particularly the sub-cellular fraction, could be a suitable early indicator of toxic effects.

These effects and the sub-cellular behaviour of cadmium were These effects and the sub-cellular behaviour of cadmium were studied with a widely used species in freshwater toxicity bioassays, Daphnia magna.

In spite of this very commonplace usage in ecotoxicological studies, very few data are available on its toxicant metabolism and in particular metal homeostasis.

Combining multi-tools analysis, a soluble protein was found: it is heat-stable, rich in sulfhydryl groups differential pulse polarography , characterised by a molecular mass of approximately 6.

Cd, Cu , and its concentration increases with Cd exposure. This evidence led us to hypothesise that metallothioneins MTs are present in D.

The increase in the Cd concentration in the surrounding medium and concomitantly in daphnids induces sub-cellular reorganisation of essential metals such as Cu and Zn.

The rate of metals in the soluble cellular fraction and associated with MTs increases with the Cd body burden. Monitoring sub-cellular distribution of metals after exposure in the natural environment could be very useful for ecotoxicological assessment.

Does cadmium pollution affect reproduction in the clam Ruditapes decussatus? A one-year case study more. View on researchgate.

Influence of salinity on trace metal Cu, Zn, Ag accumulation at the molecular, cellular and organism level in the oysterCrassostrea gigas Thunberg more.

The accumulation and physico-chemical forms of metals were determined by atomic absorption spectrometry in the whole soft tissues of oysters, by histological and microanalytical techniques in tissue sections, by gel permeation The accumulation and physico-chemical forms of metals were determined by atomic absorption spectrometry in the whole soft tissues of oysters, by histological and microanalytical techniques in tissue sections, by gel permeation chromatography of soft tissue homogenates.

Copper, zinc and silver accumulation in oysters are inversely related to salinity. Amoebocytes, which play a key role in accumulating copper and zinc in natural sea water, are able to sequester an important part of added silver as Ag2S.

In brackish water an increase of the number of amoebocytes may be considered as responsible for the enhancement of Cu and Zn concentrations in the whole soft tissues.

In such conditions, additional silver is concentrated in these cells rather than in the basement membranes which are target structures for Ag2S accumulation in sea water.

If the global fluctuations of metal concentrations in the soft tissue are ascribable mainly to changes at the histological level, the fate of metals in the soluble fraction must not be neglected since the speciation of metals influences their toxicity.

The freshening of sea water induced a change in the distribution of cytosolic silver and zinc but in no case were the molecular masses of compounds associated with each metal perfectly identical.

From these results, it is concluded that the accumulation of silver by oysters is not mediated by the same mechanisms as those for copper and zinc.

Intersite variations of a battery of biomarkers at different levels of biological organisation in the estuarine endobenthic worm Nereis diversicolor Polychaeta, Nereididae more.

Comparative study of the patterns of bioaccumulation of essential Cu, Zn and non-essential Cd, Pb trace metals in various estuarine and coastal organisms more.

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